|PIGEARD Benjamin||SAXENA Mohit||TRAN-RAJAU Jaouen||TRY Elisabeth|
School / University
Frank Yates, PhD
Combining the power of microfluidics with the potential of organoids
As miniaturized organ-like structures grown from human stem cells, organoids represent interesting research models that recall certain biological processes specific to human organs and present a similar microenvironment. Therefore, processes used to grow 3D cellular models such as organoids are currently being developed to complement the actual models, like 2D cellular culture and animal testing.
One of the biggest challenges in culturing organoids remain to increase the viability of the inner cells, due to lack of nutrients and the low oxygen levels within their core, leading to necrotic tissues. To overcome these challenges, our project aims to combine fluidic approaches to culture long-lasting organoids. We propose two different strategies to address the problem: macro-fluidic and micro-fluidic strategies.
The macro-fluidic device is an adaptation of the Spin ∞, using a different shape of impeller to obtain an optimal transfer of oxygen and a better uptake of nutrients. The advantage of the macro-fluidic device over conventional culture method will be the adaptability of the instrument to commercialised well-plates.
The micro-fluidic chip was designed on AutoCAD, inspired by the prototype of Yaqinq et al. (2018).
With these models, authors intend to compare both approaches, by adjusting the parameters such as: the flow rate, the height and width of the channels, diameter of the wells, and the shear stress. This might open new scopes in the development of mature disease models usable for drug testing, pharmacology, etc.
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